Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELNKTKVRKELRTERPSIYSFELDEIKQWLTDNGEKPFRAAQIFEWLYEKRVSSFEDMTNLSKDLREKLNTRFVLTTLKTAVKQTSQDGTMKFLFELHDGYTIETVLM-RHEYGNSVCVTTQVGCRIGCTFCASTLGGLK------RNLEAGEIVAQVVKVQK-ALDE-----------TDERVSSVVIMGIGEPFDNFNEMLAFLKIINHDKGLNIGARHITVSTSGIIPK-IYEFADQQ-MQINFAISLHAPNTEIRSRLMPINRAYKLPDLMEAVKYYINKTGRRISFEYGLFGGVNDQVEHAEELADLLEGVKC-HVNLIPVNYVPERDY-----VRTPRDQIFAFEKTLKSRGVNVTIRREQGHDIDAACGQLRAKERQDETR 2YX0 Chain:A ((11-304)) -----------------------------------------------------KITVQANPNMPKEVAELFRKQHYEIVGRHSGV-KLCHWLKKSL--TEGRFCYKQKFYGIHSHRCLQMTPVLAWCTHNCIFCWRPMENFLGTELPQPWDDPAFIVEESIKAQRKLLIGYKPKVDKKKFEEAWNPTHAAISLSGEPMLYP-YMGDLVEEFHK-RGF-----TTFIVTNGTIPERLEEMIKEDKLPTQLYVSITAPDIETYNSVNIPMIPDGWERILRFLELMRD-LPTRTVVRLTLVKGEN--MHSPEKYAKLILKARPMFVEAKAYM------NRLTINNMPSHQDIREFAEALVK--------------------------------

General information: TITO was launched using: RESULT: Template: 2YX0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1390 -153863 -110.69 -594.07 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -110.69 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_2YX0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YX0-query.scw PDB file : Tito_Scwrl_2YX0.pdb: