Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTIHITHPHHLIKEEQAKSVMALGYFDGVHLGHQKVIGTAKQIAEEKGLTLAVMTFHPHPSHVLGRDKEPKDLITPLEDKINQIEQLGTEVLYVVKFNEVFASLSPKQFIDQYII--GLNVQHAVAGFDFTYGKYGKGTMKTMPDDLDGKAGCTMVEKLTEQDKKISSSYIRTALQNGDVELANVLLGQPYFIKGIVIHGDKR-GRTIGFPTANVGLNNSYIVPPTGVYA-------VKAEVNGEV-----YNGVCNIGYKPTFYEKRPEQPSIEVNLFDFNQEVYGAAIKIEWYKRIRSERKFNGIKELTEQIEKDKQEAIRYFSNLRK 4UZF Chain:B ((17-313)) -------------------SAVTIGVFDGVHRGHQKLINATVEKAREVGAKAIMVTFDPHPVSVFLPREAPLG-ITTLAERFALAESFGIDGVLVIDFTRELSGTSPEKYV-EFLLEDTLHASHVVVGANFTFGENAAGTADSLRQICQSRLTVDVIDLLDDEGVRISSTTVREFLSEGDVARANWALGRHFYVTGPVVRGAGRGGKELGFPTANQYFHDTVALPADGVYAGWLTILPTEAPVSGNMEPEVAYAAAISVGTNPT------EQRSVESFVLDRDADLYGHDVKVEFVDHVRAMEKFDSVEQLLEVMAKDVQK----------

General information: TITO was launched using: RESULT: Template: 4UZF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1385 -154602 -111.63 -554.13 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -111.63 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_4UZF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UZF-query.scw PDB file : Tito_Scwrl_4UZF.pdb: