Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVKIFIDPGHGGSDPGAT--------------------G-NGLQEKTLTLQIALALRTILTNEYEGVSLLLSRTSDQYV--SLNDRTNAANNWGADFFLSIHVNSG--GGTGFESYIYPDVG-----APTTTYQSTIHSEVIQAVDFA-----DRGKK-TANFHVLRESAMPALLTENGFIDTVSDANKLKTSSFIQSLARGHANGLEQAFNLKKTSSSGLYKVQIGAFKVKANADSLASNAEAKGFDSIVLLKDGLYKVQIGAFSSKDNADTLAARAKNAGFDAIVILES 4LQ6 Chain:A ((4-215)) -MVVFIDPGHNGANDASIGRQVPTGRGGTKNCQASGTSTNSGYPEHTFTWETGLRLRAALNA-L-GVRTALSRGNDNALGPCVDERANMANALRPNAIVSLHADGGPASGRGFHVNYSAPPLNAIQAGPSVQFARIMRDQLQASGIPKANYIGQDGLYGRSDLAGLNLAQYPSILVELGNMKNPADSALMESAEGRQKYANALVRGVAGFLATQG----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4LQ6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 961 -69491 -72.31 -394.84 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -72.31 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_4LQ6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LQ6-query.scw PDB file : Tito_Scwrl_4LQ6.pdb: