Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKLCREVWIEVNLDAVKKNLRAIRRHIPHKSKIMAVVKANGYGHGSIEVARHALEHGASELAVASVEEGIVLRKAGITAPILVLGFTSLSCVKKSAAWNITLSAFQVDWMKEANEILEKEASANRLAIHINVDTGMGRLGVRTKEELLEVVKALKASKFLRWTGIFTHFSTADEPDTTLTKLQHEKFISFLSFLKKQGIELPTVHMCNTAAAIAFPEFSADMIRLGIGLYGLYPSAYIKQLNLVKLEPALSLKARIAYVKTMRTEPRTVSYGATYIAEPNEVIATLPIGYADGYSRALSNRGFVLHRGKRVPVAGRVTMDMIMVSLGENGEGKQGDEVVIYGKQKGAEISVDEVAEMLNTINYEVVSTLSRRIPRFYIRDGEIFKVSTPVLYV 1BD0 Chain:B ((2-377)) -NDFHRDTWAEVDLDAIYDNVENLRRLLPDDTHIMAVVKANAYGHGDVQVARTALEAGASRLAVAFLDEALALREKGIEAPILVLGASRPADAALAAQQRIALTVFRSDWLEEASALY---SGPFPIHFHLKMDTGMGRLGVKDEEETKRIVALIERHPHFVLEGLYTHFATADEVNTDYFSYQYTRFLHMLEWLPSRP---PLVHCANSAASLRFPDRTFNMVRFGIAMYGLAPSPGIKPLLPYPLKEAFSLHSRLVHVKKLQPGEK-VSYGATYTAQTEEWIGTIPIGYADGWLRRLQH-FHVLVDGQKAPIVGRICMDQCMIRL--PGPLPVGTKVTLIGRQGDEVISIDDVARHLETINYEVPCTISYRVPRIFFRHKRIMEV-------

General information: TITO was launched using: RESULT: Template: 1BD0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2142 -288971 -134.91 -768.54 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -134.91 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_1BD0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BD0-query.scw PDB file : Tito_Scwrl_1BD0.pdb: