Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQNPSKAEIKEILQRSKRIAVVGLSDRPDRTSHMVSKAMQDA-GYEIIPVNPTIDEALGVKAVSSLKEIDGPIDIVNVFRRSEQLPGVAEEFLETDAPVFWAQ-QG---------LVNEEAYQMLKEKGRTVIMDLCIKVAHAVTK 2CSU Chain:A ((2-154)) --------LDYFF-NPKGIAVIGASNDPKKLGYEVFKNLKEYKKGKVYPVNIKEEEVQGVKAYKSVKDIPDEIDLAIIVVPKRFVKDTLIQCGEKGVKGVVIITAGFGETGEEGKREEKELVEIAHKYGMRIIGPNCVGIMNTH--

General information: TITO was launched using: RESULT: Template: 2CSU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 597 -8566 -14.35 -69.08 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -14.35 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_2CSU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CSU-query.scw PDB file : Tito_Scwrl_2CSU.pdb: