Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRVLFSVIVFTAVGFTFCQSKAHALTFTVLPITQKTDQWSVKVSEAKNVKEFTR---PHKGEYQVYSLEVKNIGEKAATVDVQLYRNDPNSITRFSLFGCPDENCVKPKEDSKMLAESLNDGSPLKFNHFMLADKASELEVVIIWTQKGQEGRNLKQTFKFTEDGVN 2W0I Chain:A ((-1-313)) -------MPLQPEAQRALQQLKQKMVNYIQMKLDLERETIELVHTEPTDVAQLPSRVPRDAARYHFFLYKHTHEGDPLESVVFIYSMPGYKC---------SIKERMLYSSCKSRLLDSVEQDFHLEIAKKIEIGDGAEL------------------TAEFLDDEVH

General information: TITO was launched using: RESULT: Template: 2W0I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 572 23754 41.53 181.32 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 41.53 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.158

(partial model without unconserved sides chains): PDB file : Tito_2W0I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W0I-query.scw PDB file : Tito_Scwrl_2W0I.pdb: