Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRMSTIFLKIALVLIG-----IPILALCIFLVPKVANYSAELFPNIAYIKYLVFIYLYVTAI--PFYFALYQAFKLLSYIDKNKAFSGLSVRALKNIKYCAVTISIFYAAGMPVFYLMAEIDDAPGIIVIGLVIIFASMVIAVFAAVLQKLLKEAIDIKSENDLTV 4Q7O Chain:A ((4-136)) --MKNNIFLNLNKKSINNNHFVISIFFETIYQFET-KDTLLECFKNITTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAP--------------------SIYFSVG-------DDFNPEELIIPINLAYHYFNIAISDFLIAHPEYQKKCKEIQK----TY

General information: TITO was launched using: RESULT: Template: 4Q7O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 531 -50510 -95.12 -400.87 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -95.12 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_4Q7O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q7O-query.scw PDB file : Tito_Scwrl_4Q7O.pdb: