Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVMSKQMRFD-NSEVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNKMGVHIWDQ------------WKQEDGTIGHAYGFQ---LGKKNRSLN----GEKVDQVDYLLHQLKNNPSSRRHITMLWNPDDLDAMSLTPCVYETQWYVKHGKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVKDFYNFTIDDFKLINYKHGDKLLFEVAV 4EIN Chain:B ((26-306)) ------QYLRQVEHILRCGFKKE----------DRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIK-GSTNAKELSSKGVRIWDANGSRDFLDSLGFSARQEGDLGPVYGFQWRHFGAEYKDMDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVETIDDFKVEDFQIEGYNPHPTIKMEMA-

General information: TITO was launched using: RESULT: Template: 4EIN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1280 12862 10.05 49.28 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : 10.05 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.627

(partial model without unconserved sides chains): PDB file : Tito_4EIN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EIN-query.scw PDB file : Tito_Scwrl_4EIN.pdb: