Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGYLHDAFSLKGKTALVTGPGTGIGQGIAKALAGAGADIIGTSHTSSLSETQQLVEQEGRIFTSFTLDMSK----PEAIKDSAAELFENRQIDILVNNAGIIHREKAEDFPEENWQHVLNVNLNSLFILTQLAGRHMLKRGHG-KIINIASLLSFQGGILVPAYTASKHAVAGLTKSFANEWAASGIQVNAIAPGYISTANTKPIRDDEKRNEDILKRIPAGRWGQADDIGGTAVFLASRASDYVNGHILAVDGGWLSR 4Z9Y Chain:D ((4-253)) -----NSFDLQGKVALITGCDTGLGQGMAIGLAQAGCDIVGVNIVEP-KDTIEKVTALGRRFLSLTADMSNVSGHAELVEKAVAEF---GHVDILVNNAGIIRREDAIEFSEKNWDDVMNLNIKSVFFMSQTVARQFIKQGKGGKIINIASMLSFQGGIRVPSYTASKSAVMGVTRLMANEWAKHGINVNAIAPGYMATNNTQQLRADEERSKEILDRIPAGRWGLPQDLMGPSVFLASSASDYINGYTIAVDGGWLAR

General information: TITO was launched using: RESULT: Template: 4Z9Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1335 -46870 -35.11 -191.31 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.87 3D Compatibility (PKB) : -35.11 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.708

(partial model without unconserved sides chains): PDB file : Tito_4Z9Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z9Y-query.scw PDB file : Tito_Scwrl_4Z9Y.pdb: