Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MIRYPNGKTFQPKHSVSSQNSQKRAPSYSNRGMTLEDDLNETNKYYLTNQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAYFKQSSTTDYNGIYKGRYIDFEAKETKNKTSFPLQNFHDHQIE---HMKQVKAQDGICFVIISAFDQ--VYFLEADKLFY-FWDRKEKNGRKSIRKDELEETAYPISLGYAPRIDYISI------IEQLYFSPSSGAKG--------------------------- 1R5I Chain:D ((3-216)) SMKLRVENPKKAQ-KHFVQNLNNVV------FTNKELEDIYNLSNK--------------EETKEVLKLFKLKVNQFYRHAF---GIVNDYNGL-------LEYKEIFNMMFLKLSVVFDTQRKEANNVEQIKRNIAILDEIMAKADNDLSYFISQNKNFQELWDKAVKLTKEMKIK--LKGQKLDLRDGEVAINKVRELFGSDKNVKELWWFRSLLVKGVYLIKRYYEGDIELKTTSDFAKAVFED

General information: TITO was launched using: RESULT: Template: 1R5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 527 51560 97.84 298.03 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain D : 0.63 3D Compatibility (PKB) : 97.84 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.172

(partial model without unconserved sides chains): PDB file : Tito_1R5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1R5I-query.scw PDB file : Tito_Scwrl_1R5I.pdb: