Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDKTMKDIQAEVDRYIGQFKEGYFSPLAMMARLTEELGELAREVNHRYGEKPKKATEDDKSMEEEIGDVLFVLVCLANSLDISLEEAHDRVMHKFNTRDKDRWTRKEEGK 5IE9 Chain:B ((1-103)) MEAKTMKDMQKEVDAYIGQFKEGYFSPLAMMARLTEEMGELAREVNHYYGE---------RSIEEELGDVLFVMICMANSLNIDLETAHNIVMNKFNTRDKDR--------

General information: TITO was launched using: RESULT: Template: 5IE9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 196 -17631 -89.95 -187.56 target 2D structure prediction score : 0.85 Monomeric hydrophicity matching model chain B : 0.92 3D Compatibility (PKB) : -89.95 2D Compatibility (Sec. Struct. Predict.) : 0.85 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_5IE9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IE9-query.scw PDB file : Tito_Scwrl_5IE9.pdb: