Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------MKSAYIHIPFCEHICHYCDFNKYFIQSQPVDEYLNALEQEMINTIAKTGQPDLKTIFIGGGTPTSLSEEQLKKLMDMINRVLKPSSDLSEFAVEANPDDLSAEKLKILKEAGVNRLSFGVQTFEDDLLEKIGRVHKQKDVFTSFERAREIGFENISLDLMFGLPGQTLKHLEHSINTALSLDAEHYSVYSLIVEPKTVFYNLMQKGRLHLPPQEQEAEMYEIVMSKMEAHGIHQYEISNFAKAGMESKHNLTYWSNEQYFGFGAGAHGYIGGTRTVNVG--PVKHYIDLIAEKGFPYRDTHEVTTEEQIEEEMFLGLRKTAGVSKKRFAEKYGRSLDGLFPSVLKDLAEKGLIHNSESAVYLTHQGKLLGNEVFGAFLGEL 1R30 Chain:A ((27-338)) RPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVSTLLSIKTGACPEDCKYCPQSSR--YKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAA-WKNPHERDMPYLEQMVQGVKA-----MGLEACMTLGTLSESQAQRLANAGLDYYNHNLDT-SPEFYGNIITTRTYQERLDTLEKVRDAGI-KVCSGGIVGL-GETVKDRAGLLLQLANLPTPPESVPINMLVKV-------KGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLS----AGREQ-----MNEQTQAMCFMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQT---------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1R30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1427 58108 40.72 214.42 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 40.72 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_1R30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1R30-query.scw PDB file : Tito_Scwrl_1R30.pdb: