Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------MLLITPDELKSYSVFESVKTRPDELLKQDILEATADIILKVG----HDFSDAEYIPLPETVRLALLKL-SQFYALINGDESIIKGYTTEKIGDYSYTLGDGSSLQKPDVYALIKDY--VKPADPDLEGIEAKVRMRSI--- 4KTW Chain:A ((1-160)) MKKILAINFSTASKKGEGTGYAFRKDGQVYVGSIKAYNPKKTAWERTFDIVNAIKDIIDEFDLKGYHLAIETPIMGRNRKHSITLANCNGYFIGAIDGLVNGYTFIDNSKWCSYHL-ISGKREQRKEESLELLKATGLVDSNCKDDNIADAYNILTYCEHL

General information: TITO was launched using: RESULT: Template: 4KTW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 482 26593 55.17 204.56 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 55.17 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.219

(partial model without unconserved sides chains): PDB file : Tito_4KTW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KTW-query.scw PDB file : Tito_Scwrl_4KTW.pdb: