Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKTDKLLESLNVFIQKAEENQYKQLGEMVPDFPGKSNIPK--YVEEYEKGI---ARLLRRQHKKFLDGLKGFVSKDSEETLKALLVFF---------TQNLFAEDDFEEEFQELTEGFLQQTIEEMAEVIMDSIDPEVPFKVLSNRTINWIKDWSKKLAEIMKLNT-----HEVVEDVLTNAIENGSSIQDIELTLKDMPQFDRERARTTAIT--EVLAASSAAQHESYAQS---PAVKKKKWRHSG-------GKKNNPRENHIDLDGTV-IGVDEEFQIPGSSETCMFPRDP---KLSTRERVNCHCVLSPVVDSKILGLSPEEKEEIQREVLANME 4MT7 Chain:A ((45-381)) QNRDQMRANVINEIMST-ERHYIKHLKDICEGYLKQCRKRRDMFSDEQLKVIFGNIEDIYRFQMGFVRDLEKQYNNDDPHLSEIGPCFLEHQDGFWIYSEYCNNHLDACMELSKLMKDSRYQHFFEACRLLQQMID--IAIDGFLLTPVQKICKYPLQLAELLKYTAQDHSDYRYVAAALAVMRNVTQQINERKRRLENIDKIAQWQASVLDWEGDDILDRSSELIYTGEMAWIYQPYGRNQQRVFFLFDHQMVLCKKDLIRRDILYYKGRIDMDKYEVIDIEDGRDDDFNVSMKNAFKLHNKETEEVHLFFAKKLEEKIRWLRAFREERKMVQEDEKIG

General information: TITO was launched using: RESULT: Template: 4MT7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1064 55203 51.88 182.79 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 51.88 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.270

(partial model without unconserved sides chains): PDB file : Tito_4MT7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MT7-query.scw PDB file : Tito_Scwrl_4MT7.pdb: