Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERIYLDHAATSPMDERVLEQMIPHFSGSFGNPSSIHSFGRESRKWVDEARAQIAAEIGAAEQEIIFTSGGTEADNLAIMGTALARKDLGRHIITTKIEHHAVLHTCEKLEGDGFDITYLDVDQNGRVSAKQVKEALRDDTILVTVMYGNNEVGTVQPIEEIGELLKEHKAYFHTDAVQAFGLLPIDVKNSHIDLLSVSGHKLNGPKGTGFLYASKDVKLSPLLFGGEQERKRRAGTENVPGIVGLKEAIKLSSEERDEKNEKYQSFKAIFADTLRDAGVAFEVNGDKEHSLPHVLNLYFPGVSVEALLVNLDMAGVAVSSGSACTAGSVLPSHVLTAMFGEESDRLTSSIRISFGLGNTAEQVKTAAKHVADVVKRLT 4EB7 Chain:B ((3-373)) ----YFDYTSAKPVDERILEAMLPYMTESFGNPSSVHSYGFKAREAVQEAREKVAKLVNGGGGTVVFTSGATEANNLAIIGYAMRNARKGKHILVSAVEHMSVINPAKFLQKQGFEVEYIPVGKYGEVDVSFIDQKLRDDTILVSVQHANNEIGTIQPVEEISEVLA-GKAALHIDATASVGQIEVDVEKIGADMLTISSNDIYGPKGVGALWIRKEAKLQPVILGGGQENGLRSGSENVPSIVGFGKAAEITAMEWREEAERLRRLRDRIIDNVLKIEESY-LNGHPEKRLPNNVNVRFSYIEGESIVLSLDMAGIQASTGSACSSKTLQPSHVLMAC-GLKHEEAHGTLLLTLGRYNTDEDVDRLLEVLPGVIERL-

General information: TITO was launched using: RESULT: Template: 4EB7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2206 -183224 -83.06 -493.87 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -83.06 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_4EB7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EB7-query.scw PDB file : Tito_Scwrl_4EB7.pdb: