Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKDVSKNQEENISDTALTNELIHLLGHSRHDWMNKLQLIKGNLSLQKYDRVFEMIEEMVIDAKHESKLSNLKTPHLAFDFLTFNWKTHYMTLEYEVLGEIKDLSAYDQKLAKLMRKLFHLFDQAVSRESENHLTVSLQTDHPDRQLILYLDFHGAFADPSAFDDIRQNGYEDVDIMRFEIT--SHECLIEIGLD 1MXA Chain:? ((232-383)) -------IGGPMGDCGLTGRKIIVDTYGGMARHGGGAF--SGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETF-------GTEKVPSEQLTLLVREFFDL---------------------RPYGLIQMLDLL-----HPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK

General information: TITO was launched using: RESULT: Template: 1MXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 614 -57129 -93.04 -380.86 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -93.04 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.212

(partial model without unconserved sides chains): PDB file : Tito_1MXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MXA-query.scw PDB file : Tito_Scwrl_1MXA.pdb: