Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKPLILASQSPRRKELLDLLQLPYSIIVS---EVEEKLNRNFSPEENVQWLAK--------QKAKAVADLHPHAIVIGADTMVCLDGECLGKPQDQEEAASMLRRLSGRSHSVITAVSIQAENHSETF---YDKTEVAFWSLSEEEIWTYIETKEPMDKAGAYGIQGRGALFVKKIDGDYYSVMGLPISKTMRALRHFDIRA 4OO0 Chain:B ((39-230)) ----LYLASQSPRRQELLQQIGVRFELLLPRPDEDAEALEAELPGEAADAYVRRVTVAKAEAARARLVASGKPAAPVLVADTTVTIDGAILGKPTDADDALAMLTRLAGREHAVLTAVAV-IDASGELLPPALSRSSVRFAAASRDAYVRYVETGEPFGKAGAYAIQGRAAEFIERIDGSHSGIMGLPLFETAALLR------

General information: TITO was launched using: RESULT: Template: 4OO0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 808 -9929 -12.29 -55.78 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -12.29 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_4OO0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OO0-query.scw PDB file : Tito_Scwrl_4OO0.pdb: