Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTEKEMKQIQNEFKERMDEGELLNCMRCGF--CLPSCPTYIESGFQETHSPRGRIALMKAVADGMIEPDEDVERSLSLCLGCRACEPVCPSGVKYGQLLEEARDIIHQNKKHSLGERVMRKTAFHELFPHQNRMRSAVSLIGLYQRSGLQTAVRKSGMLRVLPEHLRTMEAVLPDVPKSKDMKHRPRFLPSIGPMKKRVAFFSGCLMDTMFLPTNNATLKLLQLAGCDIVIPPEQACCGALHGHSGEKNTGKELAKQNIAAFEALDVDAVITNAGGCGAFLTEYDHLLKDDPEWSERAAAFVQKLKDFSSVLVELDFHQMDLALETPQVVTYQDSCHL--RNVMHTSLEPRQLLKSIKGAEFKEMEKADSCCGSAGIYNIVEVEMSMKILDSKMAAVKATEAILIVTANPGCLLQMKLGIEREGLSGKVRAVHLADLLLEAAGHKTS---- 4Z9N Chain:A ((9-328)) ADTLSDVKAK--------------GFLQCGVNTGLLGFASPNDKG----EWSGFDVDYCRAVASAIFGDPTKVKFTP---LNAKERFTALQSG---------EVDVLIRNTTWT----ISRDTSLGLDFAGINYYDGQGFMINSKKLAGINSALQLSGA-SICVQAGTTTELNMADYFRANKMEYNPVVFEK---------------------------------------------------------------IEEANAAYDSGRCDAYTTDQSSLYGVRLALA-----NPDDHVILPEIISK-EPFGLTVRQGDARWADVVRWTHNALLNAEEYGITQANVEEMKKSDNPDIKRLLGAEADTKIGTDLGLDKDWVVKIIKGVGNYGEIFERNIGSGS-----------------PLKIAR-GLNA----------QWNKGGLQYGIPVR

General information: TITO was launched using: RESULT: Template: 4Z9N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1768 29235 16.54 93.70 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 16.54 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.198

(partial model without unconserved sides chains): PDB file : Tito_4Z9N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z9N-query.scw PDB file : Tito_Scwrl_4Z9N.pdb: