Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVEQIMK------RDVITLTKTDTLETAICKLKEFHIRHLPVVDEERHVIGMITDRDMKQASPSIFEENKRSLFLTRSVDSIMKKDVVCAHPLDFVEEISAVFYEHGIGCLPVVHHQKLIGILTKTDLLRTFVKLTGADQPGSQIEIKVNDITKSLAEISSLCQDLQVKILSVLVYPHDDPGVKVLVFRVKTMNPLPFLQALQRNGHHVVWPSEQRDLL 4FRY Chain:B ((9-137)) --VAQILKAKPDSGRTIYTVTKNDFVYDAIKLMAEKGIGALLVVDGD-DIAGIVTERDY--ARKVVLQE--RSSKATR-VEEIMTAKVRYVEPSQSTDECMALMTEHRMRHLPVLDGGKLIGLISIGDLVKSVI----ADQ-------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FRY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 544 3660 6.73 29.76 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : 6.73 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_4FRY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FRY-query.scw PDB file : Tito_Scwrl_4FRY.pdb: