Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNTELELLRQKADELNLQILKLINERGNVVKEIGKAKEAQGVNRFDPVRERTMLNNIIENNDGPFENSTIQHIFKEIFKAGLELQEEDHSKALLVSRKKKPEDTIVDIKGEKIGDGQQRFIVGPCAVESYEQVAEVAAAAKKQGIKILRGGAFKPRTSPYDFQGLGVEGLQILKRVADEFDLAVISEIVTPAHIEEALDYIDVIQIGARNMQNFELLKAAGAVKKPVLLKRGLAATISEFINAAEYIMSQGNDQIILCERGIRTYETATRNTLDISAVPILKQETHLPVFVDVTHSTGRRDLLLPTAKAALAIGADGVMAEVHPDPSVALSDSAQQMAIPEFEKWLNELKPMVKVNA 3NVT Chain:B ((30-374)) -----LEELRTQVDQLNIDLLELISKRANLVQ--------------------EMLNTILAANEGPFEDSTVQKLFKEIFKAGLELQ----SKALLVSRKNKKEDTIVTVKGLPIGNGEPVFVFGPCSVESYEQVAAVAESIKAKGLKLIRGGAFKPRTSPYDFQGLGLEGLKILKRVSDEYGLGVISEIVTPADIEVALDYVDVIQIGARNMQNFELLKAAGRVDKPILLKRGLSATIEEFIGAAEYIMSQGNGKIILCERGIRTYEKATRNTLDISAVPILKKETHLPVMVDVTHSTGRKDLLLPCAKAALAIEADGVMAEVHPDPAVALSDSAQQMDIPEFEEFWNAI--------

General information: TITO was launched using: RESULT: Template: 3NVT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1608 -167912 -104.42 -523.09 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.92 3D Compatibility (PKB) : -104.42 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_3NVT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NVT-query.scw PDB file : Tito_Scwrl_3NVT.pdb: