Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHDLIEKSKKHLWLPFTQMKD-YDENPLIIESGTGIKVKDINGKEYYDGFSSVWLNVHGHRKKELDDAIKKQLGKIAHSTLLGMTNVPATQLAETLIDISPKKLTRVFYSDSGAEAMEIALKMAFQYWKNIGKPEKQKFIAMKNGYHGDTIGAVSVGSIE-LFHHVYGPLMFESYKAPIPYVYRSESGDPDECRDQCLRELAQLLEEHHEEIAALSIESMVQGASGMIVMPEGYLAGVRELCTTYDVLMIVDEVATGFGRTGKMFACEHENVQPDLMAAGKGITGGYLPIAVTFATEDIYKAFYDDYENLKTFFHGHSYTGNQLGCAVALENLALFESENIVEQVAEKSKKLHFLLQDLHALPHVGDIRQLGFMCGAELVRSKETKEPYPADRRIGYKVSLKMRELGMLTRPLGDVIAFLPPLASTAEELSEMVAIMKQAIHEVTSLED 1DTY Chain:B ((1-425)) MTTDDLAFDERHIWHPYTSMTSPLPVYPVVSAEGCELILSD--GRRLVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGEITHAPAIELCRKLVAMTPQPLECVFLADSGSVAVEVAMKMALQYWDAKGE-ARDRFLTFRNGYHGDTFGAMSVCDPDNSMHSLWKGYLPENLFAPAPQ--SRMDGEWDE-RD--MVGFARLMAAHRHEIAAVIIEPIVQGAGGMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKALTGGTMTLSATLTTREVAETISDG--EAGCFMHGPTFMGNPLACAAANASLAILESGDWQQQVADIEVQLREQLAPARDAEMVADVRVLG------AIGVVETTHPVNMAALQKFFV-----EQGVWIRPFGKLIYLMPPYIILPQQLQRLTAAVNRAVQDET----

General information: TITO was launched using: RESULT: Template: 1DTY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2405 -5274 -2.19 -12.47 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -2.19 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_1DTY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DTY-query.scw PDB file : Tito_Scwrl_1DTY.pdb: