Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------MKKLVLIGGGYGNMRVLHRLLPNQLPDDVSITLIDRNPYHCLKTEYYALAAGTISDHHIRVSFPEHPR-----LDVQYGDITSIDIVQKQVLFQDREP--------------------ISYDDAIIGLGCEDKYHNVPGAPEFTYSIQTIDQSRETYQKLNNLSANA--------------TVAIVGAGLSGVELASELR------------ESRDDLNIILFDRGNLILSSFPERLSKYVQKWFEEHGVRIINRANITKVEEGVVYNHD---------DPISADAIVWTAGIQPNKVVRDL----DVEKDAQGRIVLTPHHNLPGDEHLYVVGDCASLPHAPSAQLAEAQAEQIVQILQKRWNGEALPESMPQFKLK----------GVLGSLGKKAGFGLVADRPLIGRVPRMLKSGLLWMYKHHNG---------------------------- 4G9K Chain:A ((1-471)) MKVIDPQHSDKPNVLILGSGWGAISFLKHIDTKKY----NVSIISPRSYFLFTPLLPSAPVGTVDEKSIIEPIVNFALKKKGNVTYYEAEATSINPDRNTVTIKSLSAVSQLYQPENHLGLHQAEPAEIKYDYLISAVGAEPNTFGIPGVTDYGHFLKEIPNSLEIRRTFAANLEKANLLPKGDPERRRLLSIVVVGGGPTGVEAAGELQDYVHQDLRKFLPALAEEVQIHLVEALPIVLNMFEKKLSSYAQSHLENTSIKVHLRTAVAKVEEKQLLAKTKHEDGKITEETIPYGTLIWATGNKARPVITDLFKKIPEQNSSKRGLAVNDFLQVKGSNNIFAIGDNAFAGLPPTAQVAHQEAEYLAKNFDKMAQIPNFQKIDLLFEENNFKPFKYNDLGALAYLGSERAIATIRSGKRTFYTGGGLMTFYLWRILYLSMILSARSRLKVFFDWIKLAFFKRDFFKGL

General information: TITO was launched using: RESULT: Template: 4G9K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1895 -41 -0.02 -0.12 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -0.02 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_4G9K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G9K-query.scw PDB file : Tito_Scwrl_4G9K.pdb: