Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MLVNSKEIVMKELLDRYMDQLH-------MACTCQVCQN--------DVLALSLNKVSPSYVTDFKK-----I-AYTKAELVDKQKNTAMLVIL-AESAAVVSESPSDLCQTKQEEAFIN------------------------- 3RPF Chain:A ((3-147)) AVLKIIQGALDTRELLKAYQEEACAKNFGAFCVFVGIVRKEDNIQGLSFDIYEALLKTWFEKWHHKAKDLGVVLKMAHSLGDVLIGQ--SSFLCVSMGKNRKNALELYENFIEDFKHNAPIWKYDLIHNKRIYAKERSHPLKGSGLL

General information: TITO was launched using: RESULT: Template: 3RPF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 361 24961 69.14 260.01 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 69.14 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.387

(partial model without unconserved sides chains): PDB file : Tito_3RPF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RPF-query.scw PDB file : Tito_Scwrl_3RPF.pdb: