Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MNPETMNKTLISISKWGKATGILFII--MGAITALSGAFFFLIGAVPGVLQIISGIFLMRSAREAGQMAEHNSGQSEDLMLENYAKFVKMQGIYLIVSIAVSILAIIAFFIFLMLGIADGLFSDTYSTY-------- 3ZFJ Chain:A ((12-130)) SYIDGDQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVP-----------------------YDAIISEELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLK----DAAHADNIRTKEEIKRQKQ

General information: TITO was launched using: RESULT: Template: 3ZFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 437 -10564 -24.17 -105.64 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -24.17 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.096

(partial model without unconserved sides chains): PDB file : Tito_3ZFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZFJ-query.scw PDB file : Tito_Scwrl_3ZFJ.pdb: