Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKPGAFQIAEGTITINEGREIREVTVKNTGSRSIQVGSHFHFAEANGALLFDRELAIGMRLDVPSGTSVRFEPGEQKTVSLVEIRGRKTIRGLNGMADTFIDERGKEKTLANLKQAGWMEGVIR 1E9Y Chain:A ((106-226)) LVPGELFLKNEDITINEGKKAVSVKVKNVGDRPVQIGSHFHFFEVNRCLDFDREKTFGKRLDIAAGTAVRFEPGEEKSVELIDIGGNRRIFGFNALVDRQADNESKKIALHRAKERGFHGA---

General information: TITO was launched using: RESULT: Template: 1E9Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 499 -35291 -70.72 -291.66 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -70.72 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_1E9Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E9Y-query.scw PDB file : Tito_Scwrl_1E9Y.pdb: