Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYAISSAADIWDHI-----SESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCGSPASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 2UZ3 Chain:D ((31-220)) ------IGWIEFITGPMFAGKTAELIRRLHRLEYADVKYLVFKPKIDTR-SIRNIQSRTGTSLPSVEVESAPEILNYIMSNSFNDETKVIGIDEVQFFDDRICEVANILAENGFVVIISGLDKNFKGEPFGPIAKLFTYADKITKLTAICNECGAEATHSLRKIDGKHADYNDDIVKIGCQEFYSAVCRHHHKVPNR---

General information: TITO was launched using: RESULT: Template: 2UZ3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 908 -26207 -28.86 -141.66 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : -28.86 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_2UZ3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2UZ3-query.scw PDB file : Tito_Scwrl_2UZ3.pdb: