Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPKVSVIMTSYNKSDYVAKSISSILSQTF---SDFELFIMDDNSNEETLNVIRPFL-N--DNRVRFYQSD-ISGVKERTEKTRYAALINQAIEMAEGEYITYATDDNI-YMPDRLLKMVRELDTHPEKAVIYSASKTYHLNENRDIV--K---ETV----RPAAQVTW---NAPCAIDHCSVMHRYSVLEKVKEKFGSYWDESPAFYRIGDARFFWRVNHFYPFYPLDEELDLNYITDQSIHFQLFELEKNEFVRNLPPQRNCRELRESLKKLGMG 3F1Y Chain:A ((94-309)) -LTVSAVLPSRNVADTVGGIIDEIHALNERAPLIDQILVVDADSEDGTAGVAASHGAEVYSENELMSGYGDAHG-------K-GDA-MWRALSVTRGDLVLYIDADTRDFRPQLAYGVLGPVLEVPGVRFVKAAY--RRP-------EEDGGGRVTELTAKPLFNLFYPELAGFVQPLAGEFVADRELFCSI----P-FL---TGY--AVETGIMIDVLKKVGLGAMAQVDLGERQNR--------------------------------------

General information: TITO was launched using: RESULT: Template: 3F1Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 959 -7892 -8.23 -41.76 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -8.23 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_3F1Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F1Y-query.scw PDB file : Tito_Scwrl_3F1Y.pdb: