Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAHDQELRRRAYEEVEKKEPIANSDPHRQHFHIMPPVGLLNDPNGVIYWKGSYHVFFQWQPFQTGHGAKFWGHYTTQDVVNWKREEIALAPSDWFDKNGCYSGSAVTKDDRLYLFYTGNVRD---QDGNRETYQCLAVSDDGLSFEK---KGVVARLPEGYTAHFRDPKVWEHEGTWYMVIGAQTENLKGQAVLFASDNLTEWRFLGPITGAGFNGLDDFGYMWECPDLFSLQGSDVLIVSPQGLEADGFRYQNVYQSGYFVGRLDYNKPELKHGEFTELDQGFDFYAPQTLEDDQGRRILFAWMAVPDQDEGSHPTIDCHWIHCMTLPRQLTLSGQKLIQQPLPELKAMRRNEKKIHINMHGSSGALPVEKPERTEILLE---DIHTESGFSISIRGTATFSFHKDEGIVTLERKSFDGKRTEARHCRIKDLHT--VHMFLDASSVEIFINNGEEVLSARYFPFPGNHEVTASATGKSEMNVGIWTLM 1UYP Chain:E ((3-396)) ----------------------------KPNYHFFPITGWMNDPNGLIFWKGKYHMFYQYNPRKPEWGNICWGHAVSDDLVHWRHLPVALYPDD--ETHGVFSGSAVEKDGKMFLVYT-YYRDPTHNKGEKET-QCVVMSENGLDFVKYDGNPVISKPPEEGTHAFRDPKVNRSNGEWRMVLGSGKDEKIGRVLLYTSDDLFHWKYEGAIFE------DETTKEIECPDLVRIGEKDILIYSITSTNSVLFSMGELKE-----GKLNVEKRGL-------LDHGTDFYAAQTFFGTD-RVVVIGWLQSWLRT-GLYPTKREGWNGVMSLPRELYVENNELKVKPVDELLALRK--RKVFETAKSGTFLLDV-KENSYEIVCEFSGEIELRMG---NESEEVVITKSRDELIVDTTRSGVSG--GEVRKSTVEDEATNRIRAFLDSCSVEFFFND------------------------------------

General information: TITO was launched using: RESULT: Template: 1UYP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 2083 20430 9.81 53.34 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain E : 0.75 3D Compatibility (PKB) : 9.81 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_1UYP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UYP-query.scw PDB file : Tito_Scwrl_1UYP.pdb: