Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKEKLSDHLKSEWKKIDQTAN----PSIPNQKELLHQLSQMKAEYRKKLLQEIILFVFCALMVVSAAILAFTQAPAVFIVLQVCVLAVLPILIAAEKKRHLGECEVKRG------------------------------- 4WJ7 Chain:A ((7-173)) -PDRLLSDYIEKEVKYLGQLTSIPGYLNPSSRTEILHFIDNAKR---AHQLPGHLTQEHDAVLSLSAYNVKLAWRDGEDIILRVPIHDIAAVSYVRDDAAHLVVLKTAQDEACCLVILAAESKVAAEELCCLLGQVFQVVY

General information: TITO was launched using: RESULT: Template: 4WJ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 340 2796 8.22 27.41 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 8.22 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.068

(partial model without unconserved sides chains): PDB file : Tito_4WJ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WJ7-query.scw PDB file : Tito_Scwrl_4WJ7.pdb: