Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAILGFVMMIVFMYLIMSNRLSALIALIVVPIVFALISGFGKDLGEMMIQGVTDLAPTGIMLLFAILYFGIMIDSGLFDPLIAKILSFVKGDPLKIAVGTAVLTMTISLDGDGTTTYMITIAAMLPLYKRLGMNRLVLAGIAMLGSGVM-NIIPWGGPTARV-L----ASLKL-------DTSEVFTPLIPAMIAGILWVIAVAYILGKKERKRLGVISIDHAPSSDPEAAPLKRPALQWFN---LLLTVALMAALIT--SL----------LP---LPVLFMTAFAVALMVN----YP-N----VKEQQKRISAHAGNALNVVSMVFAAGIFTGILSGTKMVDA-MAHSLVSL-IPDAMGPHL-PLITAIVSMPFTFFM-SNDAFYFGVLPIIAEAASAYGIDAAEIGRASLLGQPVHL--LSPLVPSTYLLVGMAGVS-----FGDHQKFTIKWAVGTTIVMTIAALLIGIISF 4R0C Chain:A ((70-477)) ---------------------------------------------FTTLVGNFTGFAPLG-VVLVAMLGLGVAEQSGLLSVSLASLVRRSSGGAL--V-FTVAFAGVLSSLT-VDAGYVVLIPLAGLVFQLAGRPPIAGIATAFAAVSGGFSANLLVGPVDATLAGLSTEAAHIIDPDRTVAATGNYWFIIASTFLVTGLVTL-ITR-TLTEP-RLAHANTVAD-ASVDAPQIH--SRAMKWTGLTLAILLAGLALLVLPNDAPLRHPDTGSVLGSPFIHGLVVIVALIAGICGAVYGRVSGQFRNSGAVITAMEVTMASMAGYLVLMFFAAQFVAWFNYSQLGLLLAVKGAAWLGALT-VPKVVLLLLFVVLTALINLMIGSASAKWSILAPVFIPMLMLLGISPEASQAAYRVGD-SSTNIITPLMPYFVLVLGFARRYQPETGIGTLIALMLPYSLTLLLGWS-----------

General information: TITO was launched using: RESULT: Template: 4R0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1665 -230699 -138.56 -646.22 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -138.56 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.379

(partial model without unconserved sides chains): PDB file : Tito_4R0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R0C-query.scw PDB file : Tito_Scwrl_4R0C.pdb: