Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMNWRALSQTKQDRIWSEVNKIIKWKPGSRCHHIIPPDPYRVFDISSAMSSKAGHNDVSGVLSDLETSILKAFQLGTGKNDVMYALDWQHDGYTFSPHQAMPKDEFGEWPVPVFPNGDYYFFFHQDFSWGLLGDPWKCAITVFGEELLEAIDNDPPILFRNK 1D4B Chain:A ((1-122)) MEYLSALNPSDLLRSVSNISS----EFGRRVWTSAPP-PQRPFRVCDH-KRTIRKGLTAATRQELLAKALETLLLNG-VLTLVL----EEDGTAVDSEDFFQL----------LEDDTCLMVLQSGQSW-------------------SPTRSGVLHHHHHH

General information: TITO was launched using: RESULT: Template: 1D4B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 280 -29406 -105.02 -241.03 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -105.02 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_1D4B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1D4B-query.scw PDB file : Tito_Scwrl_1D4B.pdb: