Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKRFSSFQAAQIRIARPTGQLDEIIRFYEEGLCLKRIGEFSQHNGYDGVMFGLPHADYHLEFTQYEGGSTA-PVPHPDSLLVFYVPNAVELAAITSKLKHMGYQEVES-ENPYWSNGGVTIEDPDGWRIVFMNSKGISGK 2KJZ Chain:A ((1-121)) ----MTHPDFTILY----VDNPPASTQFYKALLGVDPVESSP---TF--SLFVLAN-GMKLGLWSRHTVEPKASVTGGGGELAFRVENDAQVDETFAGWKASGVAMLQQPAKMEF-GYTFTAADPDSHRLRVYAFA-----

General information: TITO was launched using: RESULT: Template: 2KJZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 475 -28101 -59.16 -236.14 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -59.16 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_2KJZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KJZ-query.scw PDB file : Tito_Scwrl_2KJZ.pdb: