Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEKKNIYPNKEGCPVEFTLDVIGGKWKGILFYHMIDGKKRFNEFRRICPSITQRMLTLQLRELEADGIVHREVYHQVPPKVEYSLTEFGRTLEPIVLQMKEWGESNRDVLESYRSNGLVKDQQK 4A5M Chain:F ((14-110)) -------------CPVEFTLDVIGGKWKGILFYHMIDGKKRFNEFRRICPSITQRMLTLQLRELEADGIVHREVYHQVPPKVEYSLTEFGRTLEPIVLQMKEWGESNRDV---------------

General information: TITO was launched using: RESULT: Template: 4A5M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 284 -48939 -172.32 -504.53 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain F : 0.92 3D Compatibility (PKB) : -172.32 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_4A5M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4A5M-query.scw PDB file : Tito_Scwrl_4A5M.pdb: