Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNVNGLRAVMRKMDFLSYLKEEDADIICLQETKIQD----GQVDLQPEDYHVYWNYAV-KKGYSGTAVFSKQEPLQVIYGIGVEEHDQEGRVITLEFENVFVMTVYTPNSRRGLERIDYRMQWEEALLSYILELDQKKPVILCGDLNVAHQEIDLKNPKANRNNAGFSDQEREAFTRFLEAGFVDSFRHVYPDLEGAYSWWSYRAGARDRNIGWRIDYFVVSESLKEQIEDASISADVMGSDHCPVELIINI 2O3C Chain:B ((27-282)) MKITSWNVDGLRAWVKK-NGLDWVRKEDPDILCLQETKCAEKALPADITAMPEYPHKYWAGSEDKEGYSGVAMLCKTEPLNVTYGIGKEEHDKEGRVITAEFPDFFLVTAYVPNASRGLVRLDYRKTWDVDFRAYLCGLDARKPLVLCGDLNVAHQEIDLKNPKGNRKNAGFTPEEREGFTQLLEAGFTDSFRELYPDQAYAYTFWTYMMNARSKNVGWRLDYFVLSSALLPGLCDSKIRNTAMGSDHCPITLFLAV

General information: TITO was launched using: RESULT: Template: 2O3C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1426 4611 3.23 18.37 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : 3.23 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_2O3C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O3C-query.scw PDB file : Tito_Scwrl_2O3C.pdb: