Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTRRDLPQSPYLAAVTGRKPSRVPVWFMRQAGRSLPEYRALRERYS-MLAACFEPDVACEITLQPIRRY-DVDAAILFSDIVVPLRAAGVDLDIVADVGPVIADPVRTAADVAAMKPLDP-QAIQPVLVAASLLVAEL-GDVPLIGFAGAPFTLASYLVEGGPSRHHAHVKAMMLAEPASWHALMAKLTDLTIAFLVGQIDAGVDAIQVFDSWAGALSPIDYRQYVLPHSARVFAAL-GEHG---VPMTHFGVGTAELLGAMSEAVTAGERPGRGA-VVGVDWRTPLTDAAARVVPGTALQGNLDPAVVLAGWPAVERAARAVVDDGRRAVDAGAAGHIFNLGHGVLPESDPAVLADLVSLVHSL 4WSH Chain:B ((12-357)) ------LKNDRFLRALLKQPVDVTPVWMMRQAGRYLPEYRATRAKAGDFMSLCMNPELACEVTLQPLDRYPQLDAAILFSDILTIPDAMGQGLY------PRFRKVVSSLADIEALPVPDPEQDLGYVMDAVRTIRRELNGRVPLIGFSGSPWTLATYMVEGGSSKDFRKSKAMLYDNPKAMHALLDKLAQSVTSYLNGQIHAGAQAVQIFDSWGGSLSAAAYQEFSLAYMRKIVDGLIREHDGRRVPVILFTKGGGLWLESMAEV---------GAEALGLDWTCDIGSARARVGERVALQGNMDPSVLYAN-PAAIRAEVARI----LAAYGKGTGHVFNLGHGITPEVDPAHAGAFFEAVHEL

General information: TITO was launched using: RESULT: Template: 4WSH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -250872 for 2976 contacts (-84.3/contact) + 2D Compatibility (PS) -36085 + (NN) -17847 + (LL) 980 1D Compatibility (HY) -25200 + (ID) 7400 Total energy: -336424.0 ( -113.05 by residue) QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_4WSH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WSH-query.scw PDB file : Tito_Scwrl_4WSH.pdb: