Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTSPRMKWFVLLFTFVFAIGMNSFRNSFQFFMLPMADAFHADRSLISVSVSIFMITTGIVQFFVGFFIDRFSVRKIMALGAVCISASFLVLPYSPNVHVFSAIYGVLGGIGYSCAVGVTTQYFISCWFDTHKGLALAILTNANSAGLLLLSPIWAAAPYHAGWQSTYTILGIVMAAVLLPLLVFGMKHPPHAQAETVKKSYDWRGFWNVMKQSRLIHILYFGVFTCGFTMGIIDAHLVPILKD-AHVSHV--NGMMAAFGAFIIIGGLLAGWLSDLLGSRSVMLSILFFIRLLSLICLLIPILGIHHSDLWYFGFILLFGLSYTGVIPLTAASISESYQTGLIGSLLGINFFIHQVAGALSVYAGGLFFDMTHGYLLIVAVCIVFVGLSAVIELVPFLDKQKAKETHHSI 4ZP0 Chain:A ((27-367)) ---------------------------MIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLAQNIEQFTL-LRFLQGISLCFIGAVGYAAIRESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLPWEGMFVLFAALAAI-SFFGLQRAMPETATRIGEKLS-LKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGG-WPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR-LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNG--------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZP0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1512 -277740 -183.69 -821.71 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -183.69 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.268

(partial model without unconserved sides chains): PDB file : Tito_4ZP0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZP0-query.scw PDB file : Tito_Scwrl_4ZP0.pdb: