Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKKIALTLCPAIVGSLLFFTAPASAHVSV-KPAESAAGSWETYTMKVPSEKN-----------LPTTKVVLKMPKDVEFQQYEPIPGWKVSTQKHDDKSVSVT----WEATD------GGIQEGQFQQFTFVAKNPD--KAEEAAWDAYQYYKDGSIVEWTGDEDADTPHSITNITSAKQVTDEHGATKTEDDSENSGSSALDITAMVLSAAAIILSVAALVKKKRA 1CI3 Chain:M ((31-150)) ---------------------------AEIEVPQAVLPDSVFKAVVKIPYDHSVQQVQADGSKGPLNVGAVLMLPEGFTIA---PEDRIPEEMKEE---VGPSYLFQPYADDKQNIVLVGPLPGDEYEEIVFPVLSPNPATNKSVAFGKYSIH---------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1CI3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 323 -32270 -99.91 -336.15 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain M : 0.59 3D Compatibility (PKB) : -99.91 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.308

(partial model without unconserved sides chains): PDB file : Tito_1CI3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CI3-query.scw PDB file : Tito_Scwrl_1CI3.pdb: