Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MPTERKEDLHQQL---KEIEKWEKDQQKVWFWEKLSRLPFQLLDKLTPEFIQ-----KKIGKLLDEVGSFVQTGGQYLTSEK---QIIRMFQKKLPEEIFESLEDI-RKAPLPVMDEIAEGMGK---NRTNAATVQGATTGVGGVFTLAAD------------IPAVLGLSLKTLQDIAVAYGYDPKEKKER---VFIVKCLQLTSADVVGKRSIL--QELKHYDQDRTYKNVASQIQGWREVVLGYRDTFGWKKLFQIVPVAGMVFGAAANRSTLNDITETGMMLYKKRRILERLKETEREME 2G62 Chain:A ((17-324)) NLYFQSRNFIIPKKEIHTVPDMGKWKRSQAYADYIGFILTLNEGVKGKKLTFEYRVSEAIEKLVALLNTLDRWIDETPPVDQPSRFGNKAYRTWYAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRMEPAGLDDFQFLPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAEHS----NQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTS-

General information: TITO was launched using: RESULT: Template: 2G62.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1166 -91980 -78.89 -344.49 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -78.89 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_2G62.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2G62-query.scw PDB file : Tito_Scwrl_2G62.pdb: