Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTNYWIGVV-SEQHVLKGAAGGFAQLCHGKKAPLAK-MK----EGDWLIYYSPRDAYPDGKLLRSFTAIGKVKSGNIYPYQMA---PNFIPYRLDIDYYPCHKIGFYDIKSKLEFVQETKHLG----FLFRRGHFEISKKDFLTIAQAMGVNISGMAL 4RCJ Chain:A ((26-164)) --GRVFIIKSYSEDDIHRSIKYSIWCSTEHGNKRLDSAFRCMSSKGPVYLLFSV-------NGSGHFCGVAEMKSPVDYGTSAGVWSQDKWKGKFDVQWIFVKDVPNNQLR-HIR-----LENNDNKPVTNSRDTQEVPLEKAKQVLKIISSYK-----

General information: TITO was launched using: RESULT: Template: 4RCJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 590 -77855 -131.96 -617.90 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -131.96 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.225

(partial model without unconserved sides chains): PDB file : Tito_4RCJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RCJ-query.scw PDB file : Tito_Scwrl_4RCJ.pdb: