Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISKKVVLPLVFSAPFIFFFVLCIVVVMTISRENQVGDDFIGGGDGEYETVGIAPEVERFRAVFEKYARQEGV--FDQVNIIMALTMQESGGRSLDIMQSSESIGLPPNSITDPERSIEVGIKHFKKVFKQAG---GDVRLTLQAYNFGSGFIDYVKKNGGKYTKKLALDFSRLQAFKM--------------GW-KSYGDPSYVDHVMRYVKGSDKNVKPVKGSMDFYETVMKEALKYEGQPYAWGGSNPETGFDCSGLVQWSFAKAGITLPRTAQEQHGATKKISEKEATAGDLVFFGGTYE-GKAITHVGIYVGNGRMFNSNDSGIQYSDLKSGYWRDHLVSFGRIK 4FDY Chain:A ((46-338)) -------------------------------------------------GVTVSPEVLAHRPLIEKYGKEYGIEDYV--SYILAIMQVESGGTAEDVMQSSESLG-LPPNSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYELAEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSP----TEFDDETVQVIMDEALKYEGFPYVFGGASPTTSFD-SGLIQWVYDKAGISLPRVAQDQYDATQEISMEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGD-PIGYGDLSSRYWQDHLIGARRVI

General information: TITO was launched using: RESULT: Template: 4FDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1311 -132229 -100.86 -487.93 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -100.86 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_4FDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FDY-query.scw PDB file : Tito_Scwrl_4FDY.pdb: