Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANGNKQAGQRISPSLTLLLATACGIIVANLYYAQPLVGLISKAIGLSPSGAGLIVTLTQIGYVAGLLFLVPLGDIIENKKLIVVSLLLSAAALTLTAF------------------VKHGTLFLAAAFFVGLGS-IAAQVLVPFASYLASDAARGRVVGNVMSGLLLGIMLSRPISSLVAD--------IWGWNAIFALSAVVSVILAIVLSKVLPARKPTAN--TNYTAL----------------LGSMW---K-LLRTTPVLRRRAI----YHAFV-----FGAFSLFWTTVPLLLSGPDFHFSQKAIALY-ALVGIAGAVTAPIGGRLADRGLTRLATGIALGVVVVSLLLPLMIQSSSPVGITVLVAAAILLDMGVSA-NLVLSQRAIFS-LAPEIRSRLNGLFMAIFFLGGAIGSSIGGWAYASGGWSTALWIGIAFPVIALLYFITEK 4GC0 Chain:A ((52-430)) ----------------------------------------------AANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGSAWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLML-LYTVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFK--TLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYT-QAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMD---------------------------

General information: TITO was launched using: RESULT: Template: 4GC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1323 -193737 -146.44 -609.23 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -146.44 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.262

(partial model without unconserved sides chains): PDB file : Tito_4GC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GC0-query.scw PDB file : Tito_Scwrl_4GC0.pdb: