Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFKVFLLAASTIAVGLVELIVGGILPQ-IANDLDISIVSAGQLISVFALGYAVSGPLLLALTAKI-ERKRLYLIALFVFFLSNLVAYFSP-NFATLMVSRVLAAMSTGLIVVLSLTIAPKIVAPE-YRARAIGIIFMGFSSAIALGVPLGILISDS--------FGWRILFLGIGLLALISMLIISIFFERIPA-E---KMI---PFRE-------------------------------------------QLKTIGNLKIA---SSHLVTMFTLAGHYTLYAYFAPFLEETLHLS---------SFWVSICYFLFGISAVCGGPFGGALSDRLGS-FKSILLVTGSFAIIMFLLPLST-------SSMIFFLPVMVIWGLLSWSLAPAQQSYLIEIAPDSSDIQQSFNTSALQVGIALGSAIGGVVLDQTGTVVSTAWCGGSIVIIAVLFAFISLTRPVQTAKKSSL 4TPH Chain:A ((13-451)) ---SFMTVSLIELWERFGYYGMQALIVYFMVQRLGFDDSRANLVWSACAALIYVSPAIGGWVGDKILGTKRTMLLGAGILSVGYALMTVPTENTWFMFSALGVIVVGNGLFKPNAGNLVRKIYE--SKIDSAFTIYYMAVNVGSTFSMLLTPWIKDYVNAQYGNEFGWHAAFAVCCVGILVGLGNYA-LMHKSLANYGSEPDTRPVNKKSLAIVLALAALSVVASAIILEYEDVARVFVYAAGVAVLGIFFHL-----ERAGLIAALILTVQTVFFFIFYQQMSTSLALFALRNVDWDFQVFGTHLWTWSPAQFQALNPIWIMVLSPVLAW-------IAAKFALGFAVVAIGFFIYGFAGQFAVNGKTSSWVMIWGYASYSLGELLVSGLGLAMIAR--------MMGAYFVASGISQYLGGVVANFASVPQ-------------------------------------

General information: TITO was launched using: RESULT: Template: 4TPH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1629 -276990 -170.04 -852.28 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -170.04 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_4TPH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TPH-query.scw PDB file : Tito_Scwrl_4TPH.pdb: