Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENIIEINYNQTGKSKKTNEYGMREMQARAFEKRNSQYLLVKAPPASGKSRALMFIGLDKLINQGLRKVIVAVPERSIGSSFKNTDLKSYGFFENWKVDPRNNLTVGGDNSKVKSFVRFMESDDQVLICTHSTLRFAF-EKIDDKAFDNCLLAIDEFHHVSADVNSRLGELLRSIIHNSSAHIVAMTGSYFRGDSVPI----LLPEDEELFDKVTYSYYEQLDGYEYLKGFGIGYHFYQGQYTSAINEVLDTNKKTIVHIPNVNSGESTKDKYDEVGKILDMIGEVEYQDDDTGIIYVKRHSDGKTLKIADLVDDQVGRENVVAYLRNIEALDDLDIIIALGMAKEGFDWPFCEHTLTVGYRGSLTEIVQIIGRCTRDSYNKTYAQFTNLIAMPDAKDEVVTYTVNTMLKAISASLLMEQVLTPDFKFKRRRNESEKSSTTGELFVKGLKEPSTENVKKIIENDINDLKAKIMQDSQVQKTFSGEVDPKVLNKVLIPKVIQKVYPNLSDEEIEEVRQHVVLDTVMKGAKSEVVGSKEFIRMADKFINIEDLDINLIDSINPFQKAFEVLSKELNSPVLKLIQ--ETIDAKRINFDEDELVFIWPKV 2BMF Chain:A ((169-618)) MASIEDNP----------------EIEDDIFRKK--RLTIMDLHPGAGKTKRYLPAIVREAIKRGLRTLILA-PTRVVAAEMEEA-LRGLP-------------------------IRYQTGREIVDLMCHATFTMRLLSPIRVPNYN--LIIMDEAHFTDPASIAARGYISTRVEMGEAAGIFMTATPPGSRDPFPQSNAPIMDEEREIPER------SWNSGHEWVTDF---------------------KGKTVWFVPSIKAGNDIAACLRKNGKKV----------------------------------IQLSRKTFDSEYIKTRT-NDWDFVVTTDISEMGANFKA---ERVIDPRRCMKPVIL------------------------TDGEERVILAGPMPVTHSSAAQRRGRVG------RNPKNENDQYIYMGEPLENDEDCAHWKEAKMLLDN-INTPEGIIPSMFEPEREKVDAIDGEYRLRGEARKTFVDLMRR------GDLPVWLAYRVAAEG-----------INYADRRWCFDGVKNN---QILEENVEVEIWTKEGERKKLKPRWLDARIYSDPLALKEFKEFAAGRK-

General information: TITO was launched using: RESULT: Template: 2BMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2256 -118522 -52.54 -272.46 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -52.54 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.236

(partial model without unconserved sides chains): PDB file : Tito_2BMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BMF-query.scw PDB file : Tito_Scwrl_2BMF.pdb: