Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEKLAEAHPVWKEEEFGDKDAEDYIISYSMYNGSWLVWEKDGMPVAVSYHLEWS--------PSNGKPWLGTVLIDPAEEKKGHAKMIIEQISKLLR-AKHKAMFAGVPIERREWILFLSQCGFEQLKTEKDEKGKSFMILVKPLAEAAV 4E0A Chain:A ((56-153)) -----------------------------------VLVFVDEREKIGAYSVIHLVQTPLLPTMQQRKTVYISDLCVDETRRGGGIGRLIFEAIISYGKAHQVDAIELDVYDFNDRAKAFYHSLGMRCQKQTME------------------

General information: TITO was launched using: RESULT: Template: 4E0A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 308 -60188 -195.42 -676.27 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -195.42 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_4E0A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E0A-query.scw PDB file : Tito_Scwrl_4E0A.pdb: