Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MNTYHPFSLTTPSTLMIQD-----------WAQTNQNNKEVIAG-------FTTKNGGVSQKPFESLNTGLHVHDKDADVVKNREYIADMFNTDLQSWVFADQTHDNR--------VQKVTQRDRGKGAREYHTALKATDGIYTNEK-----NVFLALCFAD------CVP--LFFYDPVKSLVGVAHAGWK------------GTVKQI-----GREMVKQWTEKEGSNPSDIYAV--IGPSISGACYTVDDRVM----DAVRALPVSADLAANQTAKA--------------QYQLDLKELNRLILMDSGLASEQISVSGLCTESEPSLFYSHRRDQGKTGRMMSFIGMKEA------------------------------- 3FHC Chain:A ((9-405)) IPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNK--IVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAQ-KRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTE---VSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNAPVLMLLSTDGVLCPFYMIN

General information: TITO was launched using: RESULT: Template: 3FHC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1064 -111796 -105.07 -411.01 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -105.07 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.111

(partial model without unconserved sides chains): PDB file : Tito_3FHC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FHC-query.scw PDB file : Tito_Scwrl_3FHC.pdb: