Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKHSHYKDKKKFYKKVEQFKEFQERISVHFQNEKLLYQAFTHSSYVNEHRKKPYEDNERLEFLGDAVLELTISRFLFAKYPAMSEGDLTKLRAAIVCEPSLVSLAHELSFGDLVLLGKGEEMTGGRKRPALLADVFEAFIGALYLDQGLE---PVESFLKVY---VFPKINDGAFSHVMDFKSQLQEYVQRDGKGSLEYKISNEKGPAHNREFEAIVSLKGEPLGVGNGRSKKEAEQHAAQEALAKLQKHHTKQ 4M2Z Chain:B ((3-218)) ------------------MLEQLEKKLGYTFKDKSLLEKALTHVSY---SKKEHYET---LEFLGDALVNFFIVDLLVQYSPNKREGFLSPLKAYLISEEFFNLLAQKLELHKFIRIKR------GKINETIIGDVFEALWAAVYIDSGRDANFTRELFYKLFKEDILSAIKEGRVKK--DYKTILQEITQKRWKERPEYRLISVEGPHHKKKFIVEAKIK-EYRTLGEGKSKKEAEQRAAEELIKLLE------

General information: TITO was launched using: RESULT: Template: 4M2Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 840 -96894 -115.35 -461.40 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -115.35 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.608

(partial model without unconserved sides chains): PDB file : Tito_4M2Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M2Z-query.scw PDB file : Tito_Scwrl_4M2Z.pdb: