Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKALIYARVSTNKEQQETSLKRQEEELTAIAAENGMEVVKVISEKASGYEMDRDGVFELLDEIKNADIDVILVQDETRLGRGNAK-IALLHCIYREGVKVYTTAHRGELELSEA-DSMVLEIVSIVEEYQRKIHNMKIRRGMKRAVKNGFKPQKNLKNQHGNSGKEKIEVPISEIVRLRANKLTFAEIAATLRGFGYDVSKATVHRRFQEYIENEETAE 2GM4 Chain:B ((6-120)) -----YARVSTSQQ----SLDIQVRALK----DAGVKANRIFTDKAS----DRKGLDLLRMKVKEGD--VILVKKLDRLGRDTADMIQLIKEFDAQGVSIRFIDD--SIDTSSAMGRMVVTILSAVAQAERQ---------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2GM4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 414 -14137 -34.15 -129.69 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -34.15 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.370

(partial model without unconserved sides chains): PDB file : Tito_2GM4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GM4-query.scw PDB file : Tito_Scwrl_2GM4.pdb: