Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLYEIMLEHFAPKGSERGIFTYLLAQSDEEVYEWL------------KTDPSLSDGRAVYTPYQDNEANGKTYAIYNQSFDIVGHEKYKDRM-----IRLKGE-LNDEVELTDLYYGMTLVGWSMVKSD----IPSEQIELLKDTGISIESA- 3N9B Chain:A ((18-169)) ------LLRYCVQKHDASRLHYDFRLELDGTLKSWAVPKGPCLDPAVKRLAVQVEDHPLDYADFEGSIPQAGDVIVWDRGAWTPLDDPREGLEKGHLSFALDGEKLSGRWHLIRTNLRGKQSQWFLVKAKDGEARSLDRFDVLKERPDSVLSER

General information: TITO was launched using: RESULT: Template: 3N9B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 604 8871 14.69 70.97 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 14.69 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.111

(partial model without unconserved sides chains): PDB file : Tito_3N9B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N9B-query.scw PDB file : Tito_Scwrl_3N9B.pdb: