Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVKKLVVVSMLSSIAFVLMLLNFPFPGLPDY-----LKIDFSDVPAIIAILIY-----------GPLAGIAVEAIKNVLQYIIQGSMAGVPVGQVANFIAGTLFILPTAFLFKKL----------NSAKGLAVSLLLGTAAMTILMSILNYVLILPAYTWFLHSPALSDS------ALKTAVVAGILPFNMIKGIVITV-------VFSLIFIKLKPWIEQQRSAHIH------------------------------------------------------ 3QOE Chain:A ((2-296)) --SNDVDLIKRLGPSAMDQIMLYLAFSAMRTSGHRHGAFLDAAATAAKCAIYMTYLEQGQNLRMTGHLHHLEPKRVKAIVEEVRQALTEGKLLKMLGSQEPRYLIQFPYVWMEKYPWRPGRSRIPGTSLTSEEKRQIEQKLPSNLPDAHLITSFEFLELIEFLHKRSQEDLPKEHQMPLSEALAEHIKRRLLYSGTVTRIDSPWGMPFYALTRDQERTYIMVEDTARFFRMMRDWAEKRPNTMRVLEELDILPEKMQQAKDELDEIIRAWADPVVLQMVFGKK

General information: TITO was launched using: RESULT: Template: 3QOE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 539 -26095 -48.41 -138.80 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -48.41 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.102

(partial model without unconserved sides chains): PDB file : Tito_3QOE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QOE-query.scw PDB file : Tito_Scwrl_3QOE.pdb: