Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKWSELSIHTTHEAVEPISNILHEAGASGVVIEDPLDLIKERENVYGEIYQLDPNDYPDEGVIVKAYLPVNSFLGETVDGIKETINNLLLYNIDLGRNHITISEVNEEEWATAWKKYYHPVKISEKFTIVPTWEEYTPVHTDELIIEMDPGMAFGTGTHPTTVLCIQALERFVQKGDKVIDVGTGSGILSIAAAMLEAESVHAYDLDPVAVESARLNLKLNKVSDIAQVKQNNLLDGIE-GEHDVIVANILAEVILRFTSQAYSLLKEGGHFITSGIIGHKKQEVKEALEQAGFTIVEILSMEDWVSIIAKK 3CJT Chain:O ((53-248)) -------------------------------------------------------------------------------------------------------EVGDEDWLEAWRRDLKPA-LAPPFVVLAPWHTW---EGAEIPLVIEP----GTGHAETTRLALKALARHLRPGDKVLDLGTGSGVLAIAAEKLGGKAL-GVDIDPMVLPQAEANAKRNGVRP--RFLEGSLEAALPFGPFDLLVANLYAELHAALAPRYREALVPGGRALLTGILKDRAPLVREAMAGAGFRPLEEAAEGEWV------

General information: TITO was launched using: RESULT: Template: 3CJT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 972 -60766 -62.52 -318.14 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain O : 0.67 3D Compatibility (PKB) : -62.52 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.579

(partial model without unconserved sides chains): PDB file : Tito_3CJT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CJT-query.scw PDB file : Tito_Scwrl_3CJT.pdb: